Phospho-MKK7 (Ser271/Thr275) Recombinant antibody

Phospho-MKK7 (Ser271/Thr275) Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

5E9

Cat no : 80357-1-RR

Synonyms

JNK activating kinase 2, JNK kinase 2, JNKK 2, Jnkk2, MAP kinase kinase 7, MAP2K7, MAPK/ERK kinase 7, MAPKK 7, MAPKK7, MEK 7, MEK7, MKK7, Phospho-MKK7 (Ser271/Thr275), PRKMK7

---

Validation Data Gallery

• 

  WB analysis using 80357-1-RR

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80357-1-RR (Phospho-MKK7 (Ser271/Thr275) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  WB analysis using 80357-1-RR

  Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80357-1-RR (Phospho-MKK7 (Ser271/Thr275) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

---

Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells, Calyculin A treated HeLa cells, Calyculin A treated NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

80357-1-RR targets Phospho-MKK7 (Ser271/Thr275) in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: mitogen-activated protein kinase kinase 7
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47-52 kDa
• GenBank Accession Number: BC038295
• Gene Symbol: MKK7
• Gene ID (NCBI): 5609
• RRID: AB_2918891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dual specificity mitogen-activated protein kinase kinase 7 (MKK7), also known as MEK7 or MAP2K7, is a member of mitogen-activated kinase kinase (MAP2K) subfamily, and a key activator of c-Jun N-terminal kinase (JNK) signaling, a pathway that regulates primarily stress and inflammatory responses. MKK7 activity can be increased by either MKK7-autophosphorylation or phosphorylation of the Ser and Thr residues of the S-X-A-K-T motifs in the Kinase domain by upstream MEKK1, MEKK2, or MLK3.(PMID: 32783966, PMID: 31579105)

Publications

Species	Application	Title
mouse	WB	Mil Med Res; Targeting GPR65 alleviates hepatic inflammation and fibrosis by suppressing the JNK and NF-κB pathways; Authors - Kun Zhang
		Biomed Pharmacother; Cyy-272, an indazole derivative, effectively mitigates obese cardiomyopathy as a JNK inhibitor; Authors - Xin Liu