Phospho-MKK7 (Ser271/Thr275) Recombinant antibody, PBS Only

Phospho-MKK7 (Ser271/Thr275) Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5E9

Cat no : 80357-1-PBS

Synonyms

JNK activating kinase 2, JNK kinase 2, JNKK 2, Jnkk2, MAP kinase kinase 7, MAP2K7, MAPK/ERK kinase 7, MAPKK 7, MAPKK7, MEK 7, MEK7, MKK7, Phospho-MKK7 (Ser271/Thr275), PRKMK7

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Validation Data Gallery

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  WB analysis using 80357-1-RR (same clone as 80357-1-PBS)

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80357-1-RR (Phospho-MKK7 (Ser271/Thr275) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80357-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80357-1-RR (same clone as 80357-1-PBS)

  Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80357-1-RR (Phospho-MKK7 (Ser271/Thr275) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80357-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80357-1-PBS targets Phospho-MKK7 (Ser271/Thr275) in WB, Indirect ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: mitogen-activated protein kinase kinase 7
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47-52 kDa
• GenBank Accession Number: BC038295
• Gene Symbol: MKK7
• Gene ID (NCBI): 5609
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Dual specificity mitogen-activated protein kinase kinase 7 (MKK7), also known as MEK7 or MAP2K7, is a member of mitogen-activated kinase kinase (MAP2K) subfamily, and a key activator of c-Jun N-terminal kinase (JNK) signaling, a pathway that regulates primarily stress and inflammatory responses. MKK7 activity can be increased by either MKK7-autophosphorylation or phosphorylation of the Ser and Thr residues of the S-X-A-K-T motifs in the Kinase domain by upstream MEKK1, MEKK2, or MLK3.(PMID: 32783966, PMID: 31579105)