Phospho-JAK3 (Tyr980/981) Polyclonal antibody, PBS Only

Phospho-JAK3 (Tyr980/981) Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29101-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 29101-1-AP (same clone as 29101-1-PBS)

  Non-treated and IL-4 treated HepG2 cells were subjected to SDS PAGE followed by western blot with 29101-1-AP (Phospho-JAK3 (Tyr980/981) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin antibody as loading control. This data was developed using the same antibody clone with 29101-1-PBS in a different storage buffer formulation.

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Product Information

29101-1-PBS targets Phospho-JAK3 (Tyr980/981) in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Janus kinase 3 (a protein tyrosine kinase, leukocyte)
• Calculated Molecular Weight: 1124 aa, 125 kDa
• Observed Molecular Weight: 125 kDa
• GenBank Accession Number: BC028068
• Gene Symbol: JAK3
• Gene ID (NCBI): 3718
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

JAK3 belongs to the Janus kinase family of receptor-associated tyrosine kinases located in cytoplasm adjacent to the plasma membrane. Janus kinase family comprise four tyrosine (Tyr) kinases (JAK1, JAK2, JAK3 and Tyk2). In the cytoplasm, JAKs play a pivotal role in signal transduction via its association with type I and type II cytokine receptors, which include receptors for interleukins (ILs), interferons (IFNs), and hormones such as leptin. Interaction of a cytokine with its membrane-bound receptor complex triggers activation of receptor-bound JAKs, which phosphorylate the receptor on key cytoplasmic Tyr residues. These act as docking sites for SH2 domain-mediated interactions with STAT proteins. Receptor-bound STATs are then phosphorylated on conserved Tyr residues within their C terminal domains prior to dissociation from the receptor and formation of homo- or heterodimers. These translocate to the nucleus, bind target gene promoters, and stimulate target gene transcription. (PMID: 30948191)