Phospho-JAK3 (Tyr980/981) Polyclonal antibody

Phospho-JAK3 (Tyr980/981) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 29101-1-AP

Synonyms

JAK 3, JAK3, JAK3_HUMAN, JAKL, Janus kinase 3, L JAK, Leukocyte janus kinase, LJAK, Phospho-JAK3 (Tyr980/981), Tyrosine protein kinase JAK3

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Validation Data Gallery

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  WB analysis using 29101-1-AP

  Non-treated and IL-4 treated HepG2 cells were subjected to SDS PAGE followed by western blot with 29101-1-AP (Phospho-JAK3 (Tyr980/981) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin antibody as loading control.

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Tested Applications

• Positive WB detected in: IL-4 treated HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

29101-1-AP targets Phospho-JAK3 (Tyr980/981) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Janus kinase 3 (a protein tyrosine kinase, leukocyte)
• Calculated Molecular Weight: 1124 aa, 125 kDa
• Observed Molecular Weight: 125 kDa
• GenBank Accession Number: BC028068
• Gene Symbol: JAK3
• Gene ID (NCBI): 3718
• RRID: AB_2918234
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

JAK3 belongs to the Janus kinase family of receptor-associated tyrosine kinases located in cytoplasm adjacent to the plasma membrane. Janus kinase family comprise four tyrosine (Tyr) kinases (JAK1, JAK2, JAK3 and Tyk2). In the cytoplasm, JAKs play a pivotal role in signal transduction via its association with type I and type II cytokine receptors, which include receptors for interleukins (ILs), interferons (IFNs), and hormones such as leptin. Interaction of a cytokine with its membrane-bound receptor complex triggers activation of receptor-bound JAKs, which phosphorylate the receptor on key cytoplasmic Tyr residues. These act as docking sites for SH2 domain-mediated interactions with STAT proteins. Receptor-bound STATs are then phosphorylated on conserved Tyr residues within their C terminal domains prior to dissociation from the receptor and formation of homo- or heterodimers. These translocate to the nucleus, bind target gene promoters, and stimulate target gene transcription. (PMID: 30948191)

Publications

Species	Application	Title
human	WB	Front Immunol; Construction of disulfidptosis-based immune response prediction model with artificial intelligence and validation of the pivotal grouping oncogene c-MET in regulating T cell exhaustion; Authors - Pengping Li
mouse	WB	Int Immunopharmacol; Demethylzeylasteral alleviates inflammation and colitis via dual suppression of NF-κB and STAT3/5 by targeting IKKα/β and JAK2; Authors - Tian Wen