Phospho-Cofilin (Ser3) Polyclonal antibody

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  WB analysis using 29715-1-AP

  Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 29715-1-AP (Phospho-Cofilin (Ser3) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as a loading control.

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  IF Staining of HeLa using 29715-1-AP

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HeLa cells using Phospho-Cofilin (Ser3) antibody (29715-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Phospho-Cofilin (Ser3) Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 29715-1-AP

Publications(1)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

18 kDa phosphoprotein, CFL, CFL1, Cofilin 1, cofilin 1 (non muscle), Cofilin, non muscle isoform, p18, Phospho-Cofilin (Ser3)

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Tested Applications

• Positive WB detected in: λ phosphatase treated HeLa cells, HeLa cells
• Positive IF/ICC detected in: λ phosphatase treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

29715-1-AP targets Phospho-Cofilin (Ser3) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: cofilin 1 (non-muscle)
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC012318
• Gene Symbol: Cofilin
• Gene ID (NCBI): 1072
• RRID: AB_2935421
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P23528
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of the ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	BMC Cardiovasc Disord; Mechanism of adenosine monophosphate-activated protein kinase promoting lower extremity varicose veins development via cytoskeletal dynamics; Authors - Yongmei Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (03-05-2024)

Worked well for WB of glioma cells at 1:800 in 3% BSA

• Applications: Western Blot
• Primary Antibody Dilution: 1:800
• Cell Tissue Type: GBM