Phospho-APP (Thr668) Polyclonal antibody

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  WB analysis using 28788-1-AP

  Non-treated and nocodazole treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 28788-1-AP (Phospho-APP (Thr668) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Phospho-APP (Thr668) Polyclonal Antibody for WB, ELISA

Cat No. 28788-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

A4, AAA, ABETA, ABPP, AD1, AICD 50, AICD 57, AICD 59, AID(50), AID(57), AID(59), Amyloid beta A4 protein, amyloid P, amyloid precursor protein, Amyloid-beta precursor protein, APP, APPI, beta Amyloid, CTFgamma, CVAP, Gamma CTF(50) C31, Phospho-APP (Thr668), PN II, PN2, PreA4, Protease nexin II, S APP alpha Soluble APP beta

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Tested Applications

• Positive WB detected in: nocodazole treated SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28788-1-AP targets Phospho-APP (Thr668) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: amyloid beta (A4) precursor protein
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC065529
• Gene Symbol: APP
• Gene ID (NCBI): 351
• RRID: AB_2918202
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P05067
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Amyloid precursor protein (APP) is a membrane-spanning protein with a large extracellular domain and a much smaller intracellular domain(PMID: 25719338). APP is expressed in many tissues and concentrated in the synapses of neurons. The molecular weight of APP is 100-140 kDa due to the existence of several isoforms. The phosphorylation of APP at Thr668 is involved in the pathogenesis of Alzheimer's disease (AD). The Phospho-APP Thr668 form is neuron‐specific, highly expressed in dystrophic neurites and amyloid plaques of Alzheimer disease, and induces neurodegeneration(PMID: 27076121).