Phospho-AKT (Ser473) Monoclonal Matched Antibody Pair, PBS Only
Host / Isotype
Capture: Mouse / IgG1
Detection: Mouse / IgG1
Reactivity
Capture: human, mouse, rat
Detection: human, mouse, rat
Applications
Cytometric bead array, Sandwich ELISA
Range
Cytometric Bead Array: N/A
Sandwich ELISA: N/A
Validation Data Gallery
Tested Applications
Cytometric bead array, Sandwich ELISA
Recommended dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Product Information
MP50177-1 targets Phospho-AKT (Ser473) in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.
Range: N/A (Cytometric Bead Array); N/A (Sandwich ELISA);
Capture antibody: Phospho-AKT (Ser473) Monoclonal antibody, PBS Only (Capture) 66444-1-PBS (1C10B8). 100 μg. Concentration 1 mgl/ml.
Detection antibody: AKT Monoclonal antibody, PBS Only (Detector) 60203-2-PBS (2C5D1). 100 μg. Concentration 1 mgl/ml.
Matched pair protein standard:
Unconjugated mouse monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation.
Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)
Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.
| Catalog Number | 66444-1-PBS |
| Tested Reactivity | human, mouse, rat |
| Immunogen | Peptide |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | v-akt murine thymoma viral oncogene homolog 1 |
| Synonyms | AKT pan, AKT 1, AKT (pan), AKT, AKT1 |
| GenBank accession number | NM_005163 |
| Gene symbol | AKT1 |
| Uniprot | P31749 |
| Gene ID (NCBI) | 207 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS only |
| Storage Conditions | Store at -80°C. |
| Catalog Number | 60203-2-PBS |
| Tested Reactivity | human, mouse, rat |
| Immunogen | AKT fusion protein Ag16695 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | v-akt murine thymoma viral oncogene homolog 1 |
| Synonyms | AKT pan, AKT 1, AKT (pan), AKT1, AKT1/2/3 |
| GenBank accession number | BC000479 |
| Gene symbol | AKT1 |
| Uniprot | P31749 |
| Gene ID (NCBI) | 207 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS only |
| Storage Conditions | Store at -80°C. |