Phospho-AKT (Ser473) Recombinant antibody, PBS Only

Phospho-AKT (Ser473) Uni-rAb^TM Recombinant Antibody for Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E17

Cat no : 80455-1-PBS

Synonyms

AKT pan, AKT 1, AKT (pan), AKT, AKT1

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Validation Data Gallery

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  WB analysis of HEK-293 using 80455-1-RR (same clone as 80455-1-PBS)

  Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80455-1-RR (Phospho-AKT (Ser473) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80455-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293T using 80455-1-RR (same clone as 80455-1-PBS)

  Non-treated and IGF-1 treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 80455-1-RR (Phospho-AKT (Ser473) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80455-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 80455-1-RR (same clone as 80455-1-PBS)

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80455-1-RR (Phospho-AKT (Ser473) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80455-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 80455-1-RR (same clone as 80455-1-PBS)

  1X10^6 Calyculin A treated HEK-293 cells were intracellularly stained with 0.25 ug Anti-Human Phospho-AKT (Ser473) (80455-1-RR, Clone:2E17) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 80% MeOH. This data was developed using the same antibody clone with 80455-1-PBS in a different storage buffer formulation.

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Product Information

80455-1-PBS targets Phospho-AKT (Ser473) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: v-akt murine thymoma viral oncogene homolog 1
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: NM_005163
• Gene Symbol: AKT1
• Gene ID (NCBI): 207
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

AKT is a serine/threonine kinase and it participates in the key role of the PI3K signaling pathway. Phosphatidylinositol-3 kinase (PI3K) is the key regulator of AKT activation. The recruitment of inactive AKT protein to PIP3-rich areas of the plasma membrane results in a conformational change that exposes the activation loop of AKT. AKT's activating kinase, phosphoinositide-dependent protein kinase (PDK1) , is also recruited to PIP3 microdomains. PDK1 phosphorylates AKT on threonine 308 (Thr308) of the exposed activation loop, activating AKT and leading to a second phosphorylation of AKT at serine 473 (Ser473) by a kinase presumed to be mTORC2 that further potentiates kinase activity. Active AKT will phosphorylate various downstream protein targets that control cell growth and translational control and act to suppress apoptosis. (PMID: 31594388, PMID: 30808672)