Phantom Dye Violet 540 Viability Dye

Excitation Laser

Violet (405 nm)

Emission

537 nm

Solution in anhydrous DMSO

1 μl/test in DMSO

Application

FC

Cat no : PD00006

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Validation Data Gallery

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  Mouse thymocytes cells were heat killed at 65℃ for 10 minutes and then mixed with live mouse thymocytes cells. Cells were then stained with Phantom Dye Violet 540 Viability Dye. Viable gate is indicated.

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Product Information

Phantom Dye Violet 540 is a fixable viability dye which reacts with protein amine groups in both dead and live cells. Due to the compromised cell membrane in dead cells the dye can bind to many more proteins inside the cell, resulting in much higher fluorescence and easy differentation of Live/Dead cells. Phantom Dye Violet 540 is a water soluble violet fluorescent dye excited by the Violet laser (405 nm), emitting at 540 nm, making it an excellent addition to your multicolor panel.

Excitation Laser: Violet (405 nm)

Emission (nm): 537

Use By: 6 months from date of receipt

Solution in anhydrous DMSO: 1 μl/test in DMSO

Storage

-20°C protected from light and moisture. Stable for one year after shipment.

Protocol

Phantom Dyes Viability Staining Protocol

Application Notes

Phantom Dye Violet 540 Viability Dye can be used at 1uL/mL of cell suspension.  Concentration required for optimal performance should be determined empirically by investigator.

Cited in Article as

PD00006, Phantom Dye Violet 540 Viability Dye, Proteintech, IL, USA