Phantom Dye UV 450 Viability Dye

Excitation Laser

UV (355 nm)

Emission

450 nm

Formulation

1 μl/test in DMSO

Application

FC

Publications

1

Cat no : PD00003

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Validation Data Gallery

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  Mouse thymocytes cells were heat killed at 65℃ for 10 minutes and then mixed with live mouse thymocytes cells. Cells were then stained with Phantom Dye UV 450 Viability Dye. Viable gate is indicated.

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Product Information

Phantom Dye UV 450 is a fixable viability dye which reacts with protein amine groups in both dead and live cells. Due to the compromised cell membrane in dead cells the dye can bind to many more proteins inside the cell, resulting in much higher fluorescence and easy differentation of Live/Dead cells. Phantom Dye UV 450 is a water soluble UV fluorescent dye excited by the UV laser (355 nm) making it an excellent addition to your multicolor panel.

Excitation Laser: UV (355 nm)

Emission (nm): 450

Use By: 6 months from date of receipt

Solution in anhydrous DMSO: 1 μl/test in DMSO

Storage

-20°C protected from light and moisture. 

Protocol

Phantom Dyes Viability Staining Protocol

Application Notes

Phantom Dye UV 450 Viability Dye can be used at 1uL/mL of cell suspension.  Concentration required for optimal performance should be determined empirically by investigator. 

Cited in Article as

PD00003, Phantom Dye UV 450 Viability Dye, Proteintech, IL, USA

Publications

Application	Title
	Cell Rep; H3K9 lactylation in malignant cells facilitates CD8+ T cell dysfunction and poor immunotherapy response; Authors - Ruijie Wang