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Paxillin Polyclonal antibody

Paxillin Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 32083-1-AP

Synonyms

PXN

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Paxillin Polyclonal antibody (32083-1-AP)

    WB analysis using 32083-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32083-1-AP (Paxillin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA431 cells, COLO 320 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32083-1-AP targets Paxillin in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Paxillin fusion protein Ag33616
Full Name paxillin
Calculated Molecular Weight68kd
Observed Molecular Weight 68 kDa
GenBank Accession NumberNM_001080855
Gene Symbol Paxillin
Gene ID (NCBI) 5829
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PXN (paxillin) is a 68 kDa scaffold protein that interacts with multiple structural and signaling proteins and regulates cell adhesion, migration, proliferation, and apoptosis. PXN is thought to play an important role in tumor migration, invasion, and metastasis (21045234). PXN has been identified as a direct substrate of protein tyrosine phosphatase receptor-type T (PTPRT), a potent tumor suppressor gene. Increased phospho-PXN at tyrosine residue 88 (Y88) has been found as a common feature of human colon cancers (20133777).

Protocols

Product Specific Protocols
WB protocol for Paxillin antibody 32083-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols