PYROXD2 Recombinant antibody

PYROXD2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240984A12

Cat no : 83954-1-RR

Synonyms

C10orf33, YueF, FP3420, 240984A12

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Validation Data Gallery

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  WB analysis using 83954-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83954-1-RR (PYROXD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83954-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83954-1-RR (PYROXD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83954-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83954-1-RR against Human PYROXD2 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: HEK-293T cells, LNCaP cells, U-251 cells, A375 cells, L02 cells, Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83954-1-RR is PYROXD2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PYROXD2 fusion protein Ag14701
• Full Name: chromosome 10 open reading frame 33
• Calculated Molecular Weight: 581 aa, 63 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC006131
• Gene Symbol: PYROXD2
• Gene ID (NCBI): 84795
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pyridine Nucleotide-Disulfide Oxidoreductase Domain 2 (PYROXD2, previously called YueF, C10orf33) is a mitochondrial inner membrane/matrix-residing protein and is reported to regulate mitochondrial function. PYROXD2 is highly expressed in the cytoplasm of normal cells and tissues but is expressed at lower levels in corresponding cancer cells, including liver, lung and renal cell carcinoma and bladder cancer cells (PMID: 29048625, PMID: 35606887).