PXMP3 Polyclonal antibody

PXMP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 22163-1-AP

Synonyms

PAF 1, PAF1, Peroxin 2, Peroxisomal membrane protein 3, Peroxisome assembly factor 1, Peroxisome biogenesis factor 2, PEX2, PMP3, PMP35, PXMP3, RING finger protein 72, RNF72

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Validation Data Gallery

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  WB analysis of mouse liver using 22163-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22163-1-AP (PXMP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 22163-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22163-1-AP (PXMP3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22163-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22163-1-AP (PXMP3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 22163-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22163-1-AP (PXMP3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 22163-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22163-1-AP (PXMP3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 22163-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22163-1-AP (PXMP3 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, HeLa cells, mouse brain tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

22163-1-AP targets PXMP3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PXMP3 fusion protein Ag17815
• Full Name: peroxisomal membrane protein 3, 35kDa
• Calculated Molecular Weight: 305 aa, 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC000661
• Gene Symbol: PXMP3
• Gene ID (NCBI): 5828
• RRID: AB_2879014
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Cell Rep; Modulation of the cell membrane lipid milieu by peroxisomal β-oxidation induces Rho1 signaling to trigger inflammatory responses.; Authors - Anu S Nath