PXMP2 Polyclonal antibody

PXMP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24801-1-AP

Synonyms

Peroxisomal membrane protein 2, PMP22, PXMP2

---

Validation Data Gallery

• 

  WB analysis of rat liver using 24801-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 24801-1-AP (PXMP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 24801-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 24801-1-AP (PXMP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 24801-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24801-1-AP (PXMP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 24801-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24801-1-AP (PXMP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 24801-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24801-1-AP (PXMP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: rat liver tissue, mouse liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 24801-1-AP is PXMP2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PXMP2 fusion protein Ag20590
• Full Name: peroxisomal membrane protein 2, 22kDa
• Calculated Molecular Weight: 195 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC073997
• Gene Symbol: PXMP2
• Gene ID (NCBI): 5827
• RRID: AB_2879733
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Biochim Biophys Acta Biomembr; Deciphering the potential involvement of PXMP2 and PEX11B in hydrogen peroxide permeation across the peroxisomal membrane reveals a role for PEX11B in protein sorting.; Authors - Celien Lismont
human,mouse	WB	Nat Commun; Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA; Authors - Mirjana Malnar Črnigoj