Nectin-1/PVRL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Nectin-1/PVRL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 24713-1-AP

Synonyms

CD111, CLPED1, ED4, Herpes virus entry mediator C, Herpesvirus entry mediator C, Herpesvirus Ig like receptor, HIgR, HVEC, nectin 1, Nectin-1, OFC7, PRR, PRR1, PVRL1, PVRR, PVRR1, SK 12

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Validation Data Gallery

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  WB analysis using 24713-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24713-1-AP (Nectin-1/PVRL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 24713-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24713-1-AP (Nectin-1/PVRL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 24713-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 24713-1-AP (Nectin-1/PVRL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 24713-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24713-1-AP (Nectin-1/PVRL1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 24713-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24713-1-AP (Nectin-1/PVRL1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 24713-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 24713-1-AP (Nectin-1/PVRL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, SKOV-3 cells, SH-SY5Y cells, mouse cerebellum tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

24713-1-AP targets Nectin-1/PVRL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Nectin-1/PVRL1 fusion protein Ag20074
• Full Name: poliovirus receptor-related 1 (herpesvirus entry mediator C)
• Calculated Molecular Weight: 517 aa, 57 kDa
• Observed Molecular Weight: 90 kDa, 57 kDa
• GenBank Accession Number: BC104948
• Gene Symbol: Nectin-1
• Gene ID (NCBI): 5818
• RRID: AB_2879685
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nectins are a family of immunoglobulin-like cell adhesion molecules comprising four members, nectin-1 to nectin-4, each consisting of an extracellular region with three immunoglobulin-like loops, a single transmembrane domain and a cytoplasmic domain (PMID: 12901789, 18703497). Nectins are involved in cell adhesion, migration, polarization, morphogenesis, proliferation, differentiation, and survival. Nectin-1, also known as PVRL1, HVEC and CD111, is widely expressed in a variety of cell types including epithelial cells, neurons, and fibroblasts (PMID: 12901789). It promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Nectin-1 acts as a receptor for several alphaherpesviruses, including herpes simplex viruses (HSV) 1 and 2, porcine pseudorabies virus (PRV), and bovine herpesvirus 1 (BHV-1) (PMID: 9616127). Nectin-1 is a glycoprotein, the glycosylated form has a larger apparent molecular weight than the calculated molecular weight of 57 kDa (PMID: 12376527; 15731277).

Publications

Species	Application	Title
human	WB	PLoS Pathog; Porphyromonas gingivalis induces penetration of lipopolysaccharide and peptidoglycan through the gingival epithelium via degradation of junctional adhesion molecule 1.; Authors - Hiroki Takeuchi
human	IHC,WB	Front Oncol; High expression of nectin-1 indicates a poor prognosis and promotes metastasis in hepatocellular carcinoma; Authors - Xuequan Wang ; KD Validated
mouse	IHC	Mol Ther Oncolytics; Oncolytic activity of naturally attenuated herpes-simplex virus HF10 against an immunocompetent model of oral carcinoma.; Authors - Gaku Takano
human	IHC	Heliyon; Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study; Authors - Lingyan Chen
		J Virol; Neuropilin-1 Facilitates Pseudorabies Virus Replication and Viral Glycoprotein B Promotes Its Degradation in a Furin-Dependent Manner; Authors - Meng Chen
human	WB,IP,IF	Nature; TMEFF1 is a neuron-specific restriction factor for herpes simplex virus; Authors - Yao Dai ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Morgan (Verified Customer) (08-09-2021)

This PVRL1 worked well with the ovarian cancer cell line OV8. It was more difficult to get a clear detection in the ovarian cancer cell line OV90, but we believe a similar trend was apparent.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: OV8 and 0V90

Nectin-1/PVRL1 Antibody Western Blot validation (1:1000 dilution) in OV8 and 0V90 (Cat no:24713-1-AP)