PUF60 Monoclonal antibody, PBS Only

PUF60 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A3A1

Cat no : 68533-1-PBS

Synonyms

FBP interacting repressor, FIR, FLJ31379, PUF60, Ro binding protein 1, RoBP1, RoBPI, Siah binding protein 1, Siah BP1, SIAHBP1

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Validation Data Gallery

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  WB analysis using 68533-1-Ig (same clone as 68533-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68533-1-Ig (PUF60 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68533-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 68533-1-Ig (same clone as 68533-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 68533-1-Ig (PUF60 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68533-1-PBS in a different storage buffer formulation.

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Product Information

68533-1-PBS targets PUF60 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

• Tested Reactivity: Human, mouse, rat, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PUF60 fusion protein Ag33930
• Full Name: poly-U binding splicing factor 60KDa
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC008875
• Gene Symbol: PUF60
• Gene ID (NCBI): 22827
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Poly-U-binding factor 60(PUF60) is a splicing factor homologous to and complementary in function to U2AF, which involves in pre-mRNA splicing, a stepwise process initiated by binding. Also PUF60 involves in other nuclear processes such as apoptosis and transcription regulation. It can interact with and repress FUBP1-induced transcriptional activation via the core-TFIIH basal transcription factor. However, it has no repression activity in xeroderma pigmentosum complementation group B (XPB) cells.