PTRH2 Polyclonal antibody

PTRH2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 51006-2-AP

Synonyms

BIT1, CGI 147, peptidyl tRNA hydrolase 2, PTH 2, PTH2, PTRH2

---

Validation Data Gallery

• 

  WB analysis using 51006-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51006-2-AP (PTRH2 antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.

• 

  WB analysis of HEK-293 using 51006-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 51006-2-AP (PTRH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 51006-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 51006-2-AP (PTRH2 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of HepG2 using 51006-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 51006-2-AP (PTRH2 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IHC staining of human prostate cancer using 51006-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 51006-2-AP (PTRH2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 51006-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 51006-2-AP (PTRH2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 51006-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 51006-2-AP (PTRH2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 51006-2-AP

  Immunofluorescent analysis of HEK-293 cells using 51006-2-AP (PTRH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, Jurkat cells, MCF-7 cells, Raji cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

51006-2-AP targets PTRH2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTRH2 fusion protein Ag0839
• Full Name: peptidyl-tRNA hydrolase 2
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC006807
• Gene Symbol: PTRH2
• Gene ID (NCBI): 51651
• RRID: AB_2173280
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.