PTPRZ1 Polyclonal antibody

PTPRZ1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 55125-1-AP

Synonyms

HPTPZ, HPTPzeta, HTPZP2, phosphacan, PTP ZETA, PTP18, PTPRZ, PTPRZ1, PTPRZ2, PTPZ, R PTP zeta, R PTP zeta 2, RPTPB, RPTPbeta

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Validation Data Gallery

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  WB analysis of HepG2 using 55125-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55125-1-AP (PTPRZ1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 55125-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55125-1-AP (PTPRZ1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of rat cerebellum using 55125-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 55125-1-AP (PTPRZ1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat cerebellum using 55125-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 55125-1-AP (PTPRZ1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells
• Positive IHC detected in: rat cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 3 publications below

Product Information

55125-1-AP targets PTPRZ1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: protein tyrosine phosphatase, receptor-type, Z polypeptide 1
• Calculated Molecular Weight: 255 kDa
• Observed Molecular Weight: 163-175 kDa
• GenBank Accession Number: NM_002851
• Gene Symbol: PTPRZ1
• Gene ID (NCBI): 5803
• RRID: AB_10858795
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPRZ1, also named as HTPZP2, PTPRZ, PTPRZ2 and PTPZ, belongs to the protein-tyrosine phosphatase family and Receptor class 5 subfamily. It may be involved in the regulation of specific developmental processes in the CNS. PTPRZ1 has some isoforms with MW 254 kDa, 163 kDa and 90 kDa.

Publications

Species	Application	Title
mouse	WB	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
human	WB,IHC	J Oncol; Overexpression of PTPRZ1 Regulates p120/β-Catenin Phosphorylation to Promote Carcinogenesis of Oral Submucous Fibrosis.; Authors - Liwei Ma
human	IHC	Heliyon; IL-34 and its receptors as predictors of brain metastasis and prognosis in lung adenocarcinoma: Unveiling insights through bioinformatic and immunohistochemical investigations; Authors - Jianxiong Geng
human	IHC	Rapid Commun Mass Spectrom; Data-independent acquisition combined with liquid chromatography mass spectrometry technique to detect prognostic protein markers in type I gastric neuroendocrine neoplasm; Authors - Meng Zhang