PTPRT Polyclonal antibody

PTPRT Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 25473-1-AP

Synonyms

KIAA0283, PTPRT, R PTP T, RPTP rho, RPTPrho

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Validation Data Gallery

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  IHC staining of mouse brain using 25473-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25473-1-AP (PTPRT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25473-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25473-1-AP (PTPRT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25473-1-AP targets PTPRT in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: protein tyrosine phosphatase, receptor type, T
• Calculated Molecular Weight: 164 kDa
• GenBank Accession Number: NM_007050
• Gene Symbol: PTPRT
• Gene ID (NCBI): 11122
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPRT is a member of the type IIB receptor-type protein tyrosine phosphatase family, linked to signal transduction, cell adhesion and neurite extension. PTPRT plays a role in regulation of synaptic formation and neuronal development. It regulates synaptic function and neuronal development through interactions with neuronal adhesion molecules and the dephosphorylation of synaptic molecules. PTPRT is mainly expressed in nervous system (PMID:19816407, PMID: 25748173).