PTPRF Monoclonal antibody

PTPRF Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D12G7

Cat no : 68070-1-Ig

Synonyms

FLJ43335, FLJ45062, FLJ45567, LAR, PTPRF

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Validation Data Gallery

• 

  WB analysis using 68070-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68070-1-Ig (PTPRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 68070-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68070-1-Ig (PTPRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 68070-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 68070-1-Ig (PTPRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HSC-T6 cells, 4T1 cells, HEK293 cells, A549 cells, LNCaP cells, MCF-7 cells, U2OS cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68070-1-Ig targets PTPRF in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PTPRF fusion protein Ag25441
• Full Name: protein tyrosine phosphatase, receptor type, F
• Calculated Molecular Weight: 1898 aa, 212 kDa
• Observed Molecular Weight: 150 kDa, 213 kDa
• GenBank Accession Number: BC048768
• Gene Symbol: PTPRF
• Gene ID (NCBI): 5792
• RRID: AB_2918811
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.