PTGES2 Polyclonal antibody

• Featured Product
• KD/KO Validated

PTGES2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 10881-1-AP

Synonyms

mPGES 2, mPGE synthase-2, Membrane-associated prostaglandin E synthase-2, GBF1, EC:5.3.99.3

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Validation Data Gallery

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  WB analysis of HeLa using 10881-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10881-1-AP (PTGES2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10881-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10881-1-AP (PTGES2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10881-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10881-1-AP (PTGES2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 10881-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 10881-1-AP (PTGES2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10881-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10881-1-AP (PTGES2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 10881-1-AP

  IP result of anti-PTGES2 (IP:10881-1-AP, 4ug; Detection:10881-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of human breast cancer using 10881-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10881-1-AP (PTGES2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 10881-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10881-1-AP (PTGES2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10881-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10881-1-AP (PTGES2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 10881-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10881-1-AP (PTGES2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 10881-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10881-1-AP (PTGES2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 10881-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10881-1-AP (PTGES2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10881-1-AP

  Immunofluorescent analysis of Hela cells, using PTGES2 antibody 10881-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HepG2 using 10881-1-AP

  Immunofluorescent analysis of HepG2 cells using 10881-1-AP (PTGES2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, human stomach tissue, mouse heart tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 5 publications below

Product Information

10881-1-AP targets PTGES2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, caenorhabditis elegans
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTGES2 fusion protein Ag1329
• Full Name: prostaglandin E synthase 2
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 30-35 kDa, 45 kDa
• GenBank Accession Number: BC011613
• Gene Symbol: PTGES2
• Gene ID (NCBI): 80142
• RRID: AB_2175066
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTGES2 (Prostaglandin E synthase 2) catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form. Its predicted size is 42 kDa, but it was detected one band of 35 kDa in the prostate and two bands of 30 kDa, 50 kDa in liver by western blot (PMID:17566096). PTGES2 protein is a homotetramer, whereas two PTGES2 molecules each form a strongly interacting dimer(PMID:19268562).

Publications

Species	Application	Title
human,mouse	WB	Brain Behav Immun; Elevated β-secretase 1 expression mediates CD4+ T cell dysfunction via PGE2 signalling in Alzheimer's disease.; Authors - Linbin Dai
rat	WB	Int Immunopharmacol; Curc-mPEG454, a PEGylated curcumin derivative, as a multi-target anti-fibrotic prodrug.; Authors - Shuang Xiao
human	WB,IHC	Tumour Biol; Prostaglandin E2 (PGE2) promotes proliferation and invasion by enhancing SUMO-1 activity via EP4 receptor in endometrial cancer.; Authors - Jieqi Ke ; KD Validated
rat	WB	J Neuroimmunol; Prostaglandin E2 increases the expression of cyclooxygenase-2 in cultured rat microglia.; Authors - Takayuki Nagano
Caenorhabditis elegans	WB	Int J Radiat Biol; Coupling of oxidative stress responses to tricarboxylic acid cycle and prostaglandin E2 alterations in Caenorhabditis elegans under extremely low-frequency electromagnetic field.; Authors - Yongyan Sun
human	IHC	Oncol Lett; Prostaglandin E2 triggers cytochrome P450 17α hydroxylase overexpression via signal transducer and activator of transcription 3 phosphorylation and promotes invasion in endometrial cancer.; Authors - Jieqi Ke