PTGER4 Monoclonal antibody

PTGER4 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

4A2A12

Cat no : 66921-1-Ig

Synonyms

EP4R, EP4, 4A2A12



Tested Applications

Positive WB detected inpig brain tissue, rat brain tissue, mouse brain tissue, Jurkat cells, K-562 cells
Positive IHC detected inhuman prostate hyperplasia tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66921-1-Ig targets PTGER4 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PTGER4 fusion protein Ag19262
Full Name prostaglandin E receptor 4 (subtype EP4)
Calculated Molecular Weight 488 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC101534
Gene Symbol PTGER4
Gene ID (NCBI) 5734
RRIDAB_2882248
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTGER4 (Prostaglandin E2 receptor EP4 subtype, also known as EP4R) is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.

Protocols

Product Specific Protocols
WB protocol for PTGER4 antibody 66921-1-IgDownload protocol
IHC protocol for PTGER4 antibody 66921-1-IgDownload protocol
IF protocol for PTGER4 antibody 66921-1-IgDownload protocol
FC protocol for PTGER4 antibody 66921-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Signal Transduct Target Ther

m6A demethylase ALKBH5 is required for antibacterial innate defense by intrinsic motivation of neutrophil migration.

Authors - Yang Liu
human,mouseWB

J Mol Med (Berl)

Vav2 promotes ductus arteriosus anatomic closure via the remodeling of smooth muscle cells by Rac1 activation

Authors - Yinghui Chen
mouseIHC

Nat Commun

Post-resolution macrophages shape long-term tissue immunity and integrity in a mouse model of pneumococcal pneumonia

Authors - Karen T Feehan