PTGER4 Polyclonal antibody

PTGER4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24895-1-AP

Synonyms

EP4R, EP4



Tested Applications

Positive WB detected inrat brain tissue, HL-60 cells, mouse brain tissue
Positive IHC detected inhuman prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24895-1-AP targets PTGER4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTGER4 fusion protein Ag19217
Full Name prostaglandin E receptor 4 (subtype EP4)
Calculated Molecular Weight 488 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC101534
Gene Symbol PTGER4
Gene ID (NCBI) 5734
RRIDAB_2879782
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTGER4 (Prostaglandin E2 receptor EP4 subtype, also known as EP4R) is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.

Protocols

Product Specific Protocols
WB protocol for PTGER4 antibody 24895-1-APDownload protocol
IHC protocol for PTGER4 antibody 24895-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Clin Invest

Antibiotic pretreatment alleviates liver transplant damage in mice and humans.

Authors - Kojiro Nakamura
ratWB

J Cell Mol Med

Inhibition of prostaglandin E2 receptor 4 by lnc000908 to promote the endothelial-mesenchymal transition participation in cardiac remodelling.

Authors - Xingxing Chen
ratWB

Mol Pharm

PMID: 30109938

Authors - Huan Li
humanWB

Int J Mol Sci

Correlative Study on Impaired Prostaglandin E2 Regulation in Epicardial Adipose Tissue and its Role in Maladaptive Cardiac Remodeling via EPAC2 and ST2 Signaling in Overweight Cardiovascular Disease Subjects.

Authors - Elena Vianello
mouseWB

Biomolecules

In Silico, In Vitro, and In Vivo Analysis of Tanshinone IIA and Cryptotanshinone from Salvia miltiorrhiza as Modulators of Cyclooxygenase-2/mPGES-1/Endothelial Prostaglandin EP3 Pathway.

Authors - Anella Saviano
IF

Exp Cell Res

Prostaglandin E2 accelerated recovery of chemotherapy-induced intestinal damage by increasing expression of cyclin D.

Authors - Mengzhen Yue