PTGER3 Polyclonal antibody

PTGER3 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 14357-1-AP

Synonyms

EP3, EP3 I, EP3 II, EP3 III, EP3 IV, EP3e, PGE receptor EP3 subtype, PGE2 R, PGE2 receptor EP3 subtype, Prostanoid EP3 receptor, PTGER3

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Validation Data Gallery

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  IHC staining of human brain using 14357-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14357-1-AP (PTGER3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 14357-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14357-1-AP (PTGER3 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IF: See 2 publications below

Product Information

14357-1-AP targets PTGER3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTGER3 fusion protein Ag5711
• Full Name: prostaglandin E receptor 3 (subtype EP3)
• Calculated Molecular Weight: 43 kDa
• GenBank Accession Number: BC024229
• Gene Symbol: PTGER3
• Gene ID (NCBI): 5733
• RRID: AB_2237964
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB,IF	Oxid Med Cell Longev; Inhibition of Prostaglandin E2 Receptor EP3 Attenuates Oxidative Stress and Neuronal Apoptosis Partially by Modulating p38MAPK/FOXO3/Mul1/Mfn2 Pathway after Subarachnoid Hemorrhage in Rats; Authors - Yu Liu
rat	WB	Aging (Albany NY); Integrated transcriptome expression profiling reveals a novel lncRNA associated with L-DOPA-induced dyskinesia in a rat model of Parkinson's disease.; Authors - Chun-Lei Han
human	WB	Front Cell Dev Biol; An Advanced Systems Pharmacology Strategy Reveals AKR1B1, MMP2, PTGER3 as Key Genes in the Competing Endogenous RNA Network of Compound Kushen Injection Treating Gastric Carcinoma by Integrated Bioinformatics and Experimental Verification; Authors - Wei Zhou
human	WB	Int J Mol Sci; Correlative Study on Impaired Prostaglandin E2 Regulation in Epicardial Adipose Tissue and its Role in Maladaptive Cardiac Remodeling via EPAC2 and ST2 Signaling in Overweight Cardiovascular Disease Subjects.; Authors - Elena Vianello
mouse	WB	Biomolecules; In Silico, In Vitro, and In Vivo Analysis of Tanshinone IIA and Cryptotanshinone from Salvia miltiorrhiza as Modulators of Cyclooxygenase-2/mPGES-1/Endothelial Prostaglandin EP3 Pathway.; Authors - Anella Saviano
human	WB,IF	Acta Physiol (Oxf); c-Jun N-terminal Kinase mediates prostaglandin induced sympathoexcitation in rats with chronic heart failure by reducing GAD1 and GABRA1 expression.; Authors - Renjun Wang