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  • KD/KO Validated

PTEN Polyclonal antibody

PTEN Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10047-1-AP

Synonyms

10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1



Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, HEK-293T cells, DU 145 cells
Positive IP detected inHEK-293T cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10047-1-AP targets PTEN in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTEN fusion protein Ag0007
Full Name phosphatase and tensin homolog
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC005821
Gene Symbol PTEN
Gene ID (NCBI) 5728
RRIDAB_2174343
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTEN (also designated MMAC1), products of tumor suppressor genes, are found deleted in most human gliomas. The PTEN genes are also mutated in many other tumors, such as brain, breast, kidney and prostate cancers. PTEN is a protein tyrosine phosphatase that may terminate the signaling transduction pathways mediated by PI 3-kinase/Akt. PTEN has an apparent molecular weight of 55 kDa and it is located in the cytosol.

Protocols

Product Specific Protocols
WB protocol for PTEN antibody 10047-1-APDownload protocol
IHC protocol for PTEN antibody 10047-1-APDownload protocol
IP protocol for PTEN antibody 10047-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Oxid Med Cell Longev

Knockdown of miR-214 Alleviates Renal Interstitial Fibrosis by Targeting the Regulation of the PTEN/PI3K/AKT Signalling Pathway

Authors - DongHua Hou
humanWB

Redox Biol

Impairment of Akt activity by CYP2E1 mediated oxidative stress is involved in chronic ethanol-induced fatty liver.

Authors - Tao Zeng
ratWB,IHC

Cancers (Basel)

Astragaloside IV Inhibits the Proliferation of Human Uterine Leiomyomas by Targeting IDO1

Authors - Tiantian Qiu
humanWB

J Cell Mol Med

Knockdown of CENPK inhibits cell growth and facilitates apoptosis via PTEN-PI3K-AKT signalling pathway in gastric cancer.

Authors - Shusheng Wu
human,mouseWB,IF

iScience

Smurf1 silencing restores PTEN expression that ameliorates progression of human glioblastoma and sensitizes tumor cells to mTORC1/C2 inhibitor Torin1.

Authors - Qin Xia
  • KD Validated
humanWB,IHC

J Biomol Struct Dyn

Molecular dynamic simulation and functional analysis of pathogenic PTEN mutations in glioblastoma

Authors - Vildan Bozok Cetintas