PSMD6 Polyclonal antibody

PSMD6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12539-1-AP

Synonyms

KIAA0107, p42A, p44S10, PFAAP4, PSMD6, Rpn7, S10, SGA 113M

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Validation Data Gallery

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  WB analysis using 12539-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12539-1-AP (PSMD6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 12539-1-AP

  IP result of anti-PSMD6 (IP:12539-1-AP, 3ug; Detection:12539-1-AP 1:500) with K-562 cells lysate 1500ug.

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  IHC staining of mouse testis using 12539-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12539-1-AP (PSMD6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 12539-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12539-1-AP (PSMD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12539-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12539-1-AP (PSMD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: mouse testis tissue, mouse brain tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12539-1-AP targets PSMD6 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMD6 fusion protein Ag3251
• Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 6
• Calculated Molecular Weight: 389 aa, 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC012369
• Gene Symbol: PSMD6
• Gene ID (NCBI): 9861
• RRID: AB_2170789
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 26 S proteasome is a 2.5-MDa molecular machine that degrades ubiquitinated proteins in eukaryotic cells. It consists of a proteolytic core particle and two 19 S regulatory particles (RPs) composed of 6 ATPase (RPT) and 13 non-ATPase (RPN) subunits. PSMD6 gene encodes 26S proteasome regulatory subunit RPN7, the characteristic of this protein is not well known to date.

Publications

Species	Application	Title
human	WB	Cells; The Identification of Nuclear FMRP Isoform Iso6 Partners; Authors - Nassim Ledoux