PSMD10 Polyclonal antibody

PSMD10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12342-2-AP

Synonyms

Gankyrin, p28, p28(GANK), PSMD10

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Validation Data Gallery

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  WB analysis using 12342-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12342-2-AP (PSMD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skin using 12342-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12342-2-AP (PSMD10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 12342-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12342-2-AP (PSMD10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 12342-2-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 12342-2-AP (PSMD10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 12342-2-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 12342-2-AP (PSMD10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, HeLa cells, K-562 cells, PC-3 cells
• Positive IHC detected in: mouse skin tissue, human urothelial carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

12342-2-AP targets PSMD10 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMD10 fusion protein Ag2994
• Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 10
• Calculated Molecular Weight: 226 aa, 24 kDa
• Observed Molecular Weight: 24-28 kDa
• GenBank Accession Number: BC011960
• Gene Symbol: PSMD10
• Gene ID (NCBI): 5716
• RRID: AB_2172809
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	J Biomed Sci; Znf179 E3 ligase-mediated TDP-43 polyubiquitination is involved in TDP-43- ubiquitinated inclusions (UBI) (+)-related neurodegenerative pathology.; Authors - Yi-Chao Lee
human	WB	Appl Biochem Biotechnol; miR-188-5p and Host MALAT1 Regulate RBE Cell Migration, Invasion, and Apoptosis via Up-regulating PSMD10 in Cholangiocarcinoma; Authors - Zhaoqiang Yin
human	WB	Research (Wash D C); SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage; Authors - Fengli Wang
human,mouse	WB	Elife; A cleaved METTL3 potentiates the METTL3-WTAP interaction and breast cancer progression; Authors - Chaojun Yan