PSMC6 Polyclonal antibody

PSMC6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15839-1-AP

Synonyms

CADP44, p42, P44, Proteasome subunit p42, PSMC6, Rpt4, SUG2

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Validation Data Gallery

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  WB analysis using 15839-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15839-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15839-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15839-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 15839-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 15839-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15839-1-AP (PSMC6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15839-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15839-1-AP (PSMC6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15839-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15839-1-AP (PSMC6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15839-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15839-1-AP (PSMC6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, human testis tissue, rat liver tissue, mouse liver tissue, rat brain tissue
• Positive IHC detected in: human liver cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

15839-1-AP targets PSMC6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMC6 fusion protein Ag8589
• Full Name: proteasome (prosome, macropain) 26S subunit, ATPase, 6
• Calculated Molecular Weight: 389 aa, 44 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC005390
• Gene Symbol: PSMC6
• Gene ID (NCBI): 5706
• RRID: AB_2237797
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMC6(proteasome (prosome, macropain) 26S subunit, ATPase, 6) is also named as CADP44, MGC12520, P44, SUG2, p42 and belongs to the AAA ATPase family. It is a component of the 26S protease, which is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. The gene encodes a 44 kDa PSMC6.

Publications

Species	Application	Title
mouse	WB	J Biomed Sci; Znf179 E3 ligase-mediated TDP-43 polyubiquitination is involved in TDP-43- ubiquitinated inclusions (UBI) (+)-related neurodegenerative pathology.; Authors - Yi-Chao Lee
mouse	IHC	FASEB J; Glucose-induced decrease of cystathionine β-synthase mediates renal injuries.; Authors - Yanting Yu
rat	WB	Cardiovasc Drugs Ther; Quercetin Prevents In Vivo and In Vitro Myocardial Hypertrophy Through the Proteasome-GSK-3 Pathway.; Authors - Kuixiang Chen
mouse,rat	WB	Acta Pharmacol Sin; Zonisamide attenuates pressure overload-induced myocardial hypertrophy in mice through proteasome inhibition; Authors - Qian Wu