PSMC4 Polyclonal antibody

PSMC4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 11389-1-AP

Synonyms

Tat-binding protein 7, S6, MIP224, MB67-interacting protein, 26S proteasome regulatory subunit 6B

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Validation Data Gallery

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  WB analysis using 11389-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11389-1-AP (PSMC4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11389-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11389-1-AP (PSMC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11389-1-AP

  Immunofluorescent analysis of HepG2 cells, using PSMC4 antibody 11389-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of SH-SY5Y using 11389-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PSMC4 antibody (11389-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11389-1-AP targets PSMC4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMC4 fusion protein Ag1943
• Full Name: proteasome (prosome, macropain) 26S subunit, ATPase, 4
• Calculated Molecular Weight: 418 aa, 47 kDa
• Observed Molecular Weight: 43-50 kDa
• GenBank Accession Number: BC014488
• Gene Symbol: PSMC4
• Gene ID (NCBI): 5704
• RRID: AB_2300373
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMC4(proteasome (prosome, macropain) 26S subunit, ATPase, 4) is also named as TBP7, S6, TBP7, MIP224 and belongs to the AAA ATPase family. The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The predicted 458-amino acid TBP7 protein is 57% identical to TBP1 and they can form heterodimers(PMID:8419915).

Publications

Species	Application	Title
mouse	IF	Neuron; Antibody Therapy Targeting RAN Proteins Rescues C9 ALS/FTD Phenotypes in C9orf72 Mouse Model.; Authors - Lien Nguyen
human	WB	Nat Commun; The 20S as a stand-alone proteasome in cells can degrade the ubiquitin tag.; Authors - Indrajit Sahu
rat	WB	Nutrients; Role of Epigallocatechin Gallate in Glucose, Lipid, and Protein Metabolism and L-Theanine in the Metabolism-Regulatory Effects of Epigallocatechin Gallate.; Authors - Ling Lin
human	IHC	Biosci Rep; A novel role of proteasomal β1 subunit in tumorigenesis.; Authors - Yuan Fuqiang F
rat	WB	Cells; The Prostaglandin E2 Receptor EP4 Promotes Vascular Neointimal Hyperplasia through Translational Control of Tenascin C via the cAPM/PKA/mTORC1/rpS6 Pathway; Authors - Hu Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tatyana (Verified Customer) (03-11-2023)

Was tested in ICC on PFA-fixed human cells. Provides a signal that looks specific (as compared to no primary control). 4% PFA followed by methanol permebealisation; antibody prepared in 5% goat serum in PBST, overnight incubation (no blocking step).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human, SH-SY5Y cells

PSMC4 Antibody Immunofluorescence validation (1:1000 dilution) in Human, SH-SY5Y cells (Cat no:11389-1-AP)