PSMB5 Monoclonal antibody, PBS Only

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  WB analysis using 67959-1-Ig (same clone as 67959-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67959-1-Ig (PSMB5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67959-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67959-1-Ig (same clone as 67959-1-PBS)

  WB result of PSMB5 antibody (67959-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMB5 transfected HeLa cells. This data was developed using the same antibody clone with 67959-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67959-1-Ig (same clone as 67959-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67959-1-Ig (PSMB5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67959-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67959-1-Ig (same clone as 67959-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67959-1-Ig (PSMB5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67959-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67959-1-Ig (same clone as 67959-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMB5 antibody (67959-1-Ig, Clone: 2E9H1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67959-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 67959-1-Ig (same clone as 67959-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PSMB5 Monoclonal antibody (67959-1-Ig, Clone:2E9H1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67959-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PSMB5 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 67959-1-PBS

Clone No.2E9H1

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

X, Proteasome subunit beta type-5, MB1, LMPX, EC:3.4.25.1

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Product Information

67959-1-PBS targets PSMB5 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5828

  Product name: Recombinant human PSMB5 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-263 aa of BC057840

  Sequence: MALASVLERPLPVNQRGFFGLGGRADLLDLGPGSLSDGLSLAAPGWGVPEEPGIEMLHGTTTLAFKFRHGVIVAADSRATAGAYIASQTVKKVIEINPYLLGTMAGGAADCSFWERLLARQCRIYELRNKERISVAAASKLLANMVYQYKGMGLSMGTMICGWDKRGPGLYYVDSEGNRISGATFSVGSGSVYAYGVMDRGYSYDLEVEQAYDLARRAIYQATYRDAYSGGAVNLYHVREDGWIRVSSDNVADLHEKYSGSTP

• Full Name: proteasome (prosome, macropain) subunit, beta type, 5
• Calculated Molecular Weight: 263 aa, 28 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC057840
• Gene Symbol: PSMB5
• Gene ID (NCBI): 5693
• RRID: AB_2918710
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P28074
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PSMB5(Proteasome subunit beta type-5) is also named as LMPX, MB1, X and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.RNAi-mediated silencing of.It has 2 isoforms produced by alternative splicing.