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PSMB5 Polyclonal antibody

PSMB5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19178-1-AP

Synonyms

X, Proteasome subunit beta type-5, MB1, LMPX, EC:3.4.25.1



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, L02 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19178-1-AP targets PSMB5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMB5 fusion protein Ag5828
Full Name proteasome (prosome, macropain) subunit, beta type, 5
Calculated Molecular Weight 263 aa, 28 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC057840
Gene Symbol PSMB5
Gene ID (NCBI) 5693
RRIDAB_10926385
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMB5(Proteasome subunit beta type-5) is also named as LMPX, MB1, X and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.RNAi-mediated silencing of.It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PSMB5 antibody 19178-1-APDownload protocol
IHC protocol for PSMB5 antibody 19178-1-APDownload protocol
IF protocol for PSMB5 antibody 19178-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Cancer vulnerabilities unveiled by genomic loss.

Authors - Nijhawan Deepak D
humanWB

Nat Commun

The deubiquitinase OTUD1 regulates immunoglobulin production and proteasome inhibitor sensitivity in multiple myeloma

Authors - Alexander Vdovin
humanIF,WB

Cancer Lett

Combinations of ivermectin with proteasome inhibitors induce synergistic lethality in multiple myeloma

Authors - Hongmei Luo
mouseWB

Front Pharmacol

Selective Inhibition of the Immunoproteasome β5i Prevents PTEN Degradation and Attenuates Cardiac Hypertrophy.

Authors - Xin Xie
humanWB

J Cancer

Cytoplasm-localized SIRT1 downregulation attenuates apoptosis and cell cycle arrest in cisplatin-resistant lung cancer A549 cells.

Authors - Hyeran Yu
mouseWB

Biochem Pharmacol

Melatonin inhibits angiotensin II-induced atrial fibrillation through preventing degradation of Ang II Type I Receptor-Associated Protein (ATRAP)

Authors - Xin Xie