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PSMB5 Polyclonal antibody
PSMB5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 19178-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, L02 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 12 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
19178-1-AP targets PSMB5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PSMB5 fusion protein Ag5828 |
Full Name | proteasome (prosome, macropain) subunit, beta type, 5 |
Calculated Molecular Weight | 263 aa, 28 kDa |
Observed Molecular Weight | 22 kDa |
GenBank Accession Number | BC057840 |
Gene Symbol | PSMB5 |
Gene ID (NCBI) | 5693 |
RRID | AB_10926385 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PSMB5(Proteasome subunit beta type-5) is also named as LMPX, MB1, X and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.RNAi-mediated silencing of.It has 2 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
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WB protocol for PSMB5 antibody 19178-1-AP | Download protocol |
IHC protocol for PSMB5 antibody 19178-1-AP | Download protocol |
IF protocol for PSMB5 antibody 19178-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun The deubiquitinase OTUD1 regulates immunoglobulin production and proteasome inhibitor sensitivity in multiple myeloma | ||
Cancer Lett Combinations of ivermectin with proteasome inhibitors induce synergistic lethality in multiple myeloma | ||
Front Pharmacol Selective Inhibition of the Immunoproteasome β5i Prevents PTEN Degradation and Attenuates Cardiac Hypertrophy. | ||
J Cancer Cytoplasm-localized SIRT1 downregulation attenuates apoptosis and cell cycle arrest in cisplatin-resistant lung cancer A549 cells. | ||
Biochem Pharmacol Melatonin inhibits angiotensin II-induced atrial fibrillation through preventing degradation of Ang II Type I Receptor-Associated Protein (ATRAP) |