PSMB5 Polyclonal antibody

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• PSMB5 Polyclonal antibody (19178-1-AP)

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PSMB5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19178-1-AP

Publications(22)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EC:3.4.25.1, LMPX, MB1, Proteasome subunit beta type-5, X

• 

  WB analysis using 19178-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19178-1-AP (PSMB5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 19178-1-AP

  WB result of PSMB5 antibody (19178-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMB5 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 19178-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19178-1-AP (PSMB5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 19178-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19178-1-AP (PSMB5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human thyroid cancer using 19178-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 19178-1-AP (PSMB5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human thyroid cancer using 19178-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 19178-1-AP (PSMB5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 19178-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19178-1-AP (PSMB5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 19178-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19178-1-AP (PSMB5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 19178-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19178-1-AP (PSMB5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat testis using 19178-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 19178-1-AP (PSMB5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat testis using 19178-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 19178-1-AP (PSMB5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 19178-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 19178-1-AP (PSMB5 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, L02 cells
• Positive IHC detected in: human thyroid cancer tissue, human breast cancer tissue, mouse brain tissue, mouse testis tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19178-1-AP targets PSMB5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5828

  Product name: Recombinant human PSMB5 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-263 aa of BC057840

  Sequence: MALASVLERPLPVNQRGFFGLGGRADLLDLGPGSLSDGLSLAAPGWGVPEEPGIEMLHGTTTLAFKFRHGVIVAADSRATAGAYIASQTVKKVIEINPYLLGTMAGGAADCSFWERLLARQCRIYELRNKERISVAAASKLLANMVYQYKGMGLSMGTMICGWDKRGPGLYYVDSEGNRISGATFSVGSGSVYAYGVMDRGYSYDLEVEQAYDLARRAIYQATYRDAYSGGAVNLYHVREDGWIRVSSDNVADLHEKYSGSTP

• Full Name: proteasome (prosome, macropain) subunit, beta type, 5
• Calculated Molecular Weight: 263 aa, 28 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC057840
• Gene Symbol: PSMB5
• Gene ID (NCBI): 5693
• RRID: AB_10926385
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P28074
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMB5(Proteasome subunit beta type-5) is also named as LMPX, MB1, X and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.RNAi-mediated silencing of.It has 2 isoforms produced by alternative splicing.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell; Cancer vulnerabilities unveiled by genomic loss.; Authors - Nijhawan Deepak D
human	WB	Nat Commun; The deubiquitinase OTUD1 regulates immunoglobulin production and proteasome inhibitor sensitivity in multiple myeloma; Authors - Alexander Vdovin
human	WB,IF	Cancer Lett; Combinations of ivermectin with proteasome inhibitors induce synergistic lethality in multiple myeloma; Authors - Hongmei Luo
mouse	WB	Front Pharmacol; Selective Inhibition of the Immunoproteasome β5i Prevents PTEN Degradation and Attenuates Cardiac Hypertrophy.; Authors - Xin Xie
human	WB	J Cancer; Cytoplasm-localized SIRT1 downregulation attenuates apoptosis and cell cycle arrest in cisplatin-resistant lung cancer A549 cells.; Authors - Hyeran Yu
		Acta Pharmacol Sin; Inhibition of CDC27 O-GlcNAcylation coordinates the antitumor efficacy in multiple myeloma through the autophagy-lysosome pathway; Authors - Hai-Qi Wu