PSMA1 Polyclonal antibody

PSMA1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11175-1-AP

Synonyms

20S proteasome, 30 kDa prosomal protein, HC2, Macropain subunit C2, NU, PROS 30, PROS30, Proteasome component C2, Proteasome nu chain, PSC2, PSMA1

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Validation Data Gallery

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  WB analysis of COLO 320 using 11175-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11175-1-AP (PSMA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 11175-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11175-1-AP (PSMA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11175-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11175-1-AP (PSMA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11175-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11175-1-AP (PSMA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11175-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11175-1-AP (PSMA1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of PC-3 using 11175-1-AP

  Immunofluorescent analysis of PC-3 cells using 11175-1-AP (PSMA1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: COLO 320 cells, HeLa cells, mouse kidney tissue, PC-3 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11175-1-AP targets PSMA1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMA1 fusion protein Ag1660
• Full Name: proteasome (prosome, macropain) subunit, alpha type, 1
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 29-33 kDa
• GenBank Accession Number: BC015105
• Gene Symbol: PSMA1
• Gene ID (NCBI): 5682
• RRID: AB_2171377
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMA1(Proteasome subunit alpha type-1) is also named as HC2, NU, PROS30, PSC2 and belongs to the peptidase T1A family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It is induced in breast cancer tissue and up-regulated in liver tumor tissues(PMID:17127214;16317774;17004105).

Publications

Species	Application	Title
rat	WB	Int J Mol Sci; Impaired 26S Proteasome Assembly Precedes Neuronal Loss in Mutant UBQLN2 Rats.; Authors - Wenjuan Zhang