PSAP Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PSAP Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F11B2

Cat no : 66473-1-PBS

Synonyms

A1 activator, Cerebroside sulfate activator, Co beta glucosidase, Component C, CSAct, Dispersin, GLBA, Glucosylceramidase activator, Proactivator polypeptide, prosaposin, Protein C, PSAP, SAP 1, SAP 2 Saposin D, SAP1

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Validation Data Gallery

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  WB analysis of human milk using 66473-1-Ig (same clone as 66473-1-PBS)

  human milk were subjected to SDS PAGE followed by western blot with 66473-1-Ig (PSAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66473-1-Ig (same clone as 66473-1-PBS)

  WB result of PSAP antibody (66473-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HeLa cells. This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66473-1-Ig (same clone as 66473-1-PBS)

  WB result of PSAP antibody (66473-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HepG2 cells. This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66473-1-Ig (same clone as 66473-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66473-1-Ig (PSAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 66473-1-Ig (same clone as 66473-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66473-1-Ig (PSAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66473-1-Ig (same clone as 66473-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66473-1-Ig (PSAP antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66473-1-Ig (same clone as 66473-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66473-1-Ig (PSAP antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human prostate cancer using 66473-1-Ig (same clone as 66473-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using PSAP antibody (66473-1-Ig, Clone: 2F11B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human prostate cancer using 66473-1-Ig (same clone as 66473-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using PSAP antibody (66473-1-Ig, Clone: 2F11B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66473-1-PBS targets PSAP in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PSAP fusion protein Ag1194
• Full Name: prosaposin
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC001503
• Gene Symbol: PSAP
• Gene ID (NCBI): 5660
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.