PRTFDC1 Polyclonal antibody

PRTFDC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11986-1-AP

Synonyms

Phosphoribosyltransferase domain-containing protein 1, HHGP

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Validation Data Gallery

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  WB analysis of fetal human brain using 11986-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11986-1-AP (PRTFDC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 11986-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 11986-1-AP (PRTFDC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11986-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11986-1-AP (PRTFDC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11986-1-AP

  IP result of anti-PRTFDC1 (IP:11986-1-AP, 4ug; Detection:11986-1-AP 1:600) with mouse brain tissue lysate 4000ug.

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  IHC staining of human ovary tumor using 11986-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11986-1-AP (PRTFDC1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 11986-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11986-1-AP (PRTFDC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, human brain tissue, Y79 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11986-1-AP targets PRTFDC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRTFDC1 fusion protein Ag2614
• Full Name: phosphoribosyl transferase domain containing 1
• Calculated Molecular Weight: 225 aa, 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC008662
• Gene Symbol: PRTFDC1
• Gene ID (NCBI): 56952
• RRID: AB_2284509
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	PLoS One; PRTFDC1 is a genetic modifier of HPRT-deficiency in the mouse.; Authors - Keebaugh Alaine C AC