PROX1 Monoclonal antibody, PBS Only

• 

  WB analysis of HuH-7 using 67438-1-Ig (same clone as 67438-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 67438-1-Ig (PROX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 67438-1-Ig (same clone as 67438-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67438-1-Ig (PROX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67438-1-Ig (same clone as 67438-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67438-1-Ig (PROX1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67438-1-Ig (same clone as 67438-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67438-1-Ig (PROX1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HuH-7 using 67438-1-Ig (same clone as 67438-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using PROX1 antibody (67438-1-Ig, Clone: 1D4B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

PROX1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67438-1-PBS

Clone No.1D4B1

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PROX-1, Prospero homeobox protein 1, prospero homeobox 1, Homeobox prospero-like protein PROX1, 1D4B1

---

Product Information

67438-1-PBS targets PROX1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PROX1 fusion protein Ag9681
• Full Name: prospero homeobox 1
• Calculated Molecular Weight: 737 aa, 83 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC024201
• Gene Symbol: PROX1
• Gene ID (NCBI): 5629
• RRID: AB_2882674
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q92786
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PROX1, Prospero homeobox protein 1, belongs to the Prospero homeobox family and contain a Prospero-type homeobox DNA-binding domain. PROX1 plays a fundamental role in early development of CNS and regulates the expression of gene and development of postmitotic, undifferentiated neurons. Also, PROX1 is a required regulator of the development of the lymphatic system. This antibody is raised against part of C-terminal chain of human PROX1. The calculated molecular weight of PROX1 is 83 kDa, but with post-translational modifications, PROX1 is about 90 kDa.