PROSC Recombinant antibody

PROSC Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240998B6

Cat no : 83831-5-RR

Synonyms

PLPBP, Pyridoxal phosphate-binding protein, Pyridoxal phosphate homeostasis protein, Proline synthase co-transcribed bacterial homolog protein, PLP homeostasis protein

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Validation Data Gallery

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  WB analysis using 83831-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83831-5-RR (PROSC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83831-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83831-5-RR against Human PROSC were performed. The affinity constant is 2.00 nM.

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Tested Applications

• Positive WB detected in: HepG2 cells, K-562 cells, Jurkat cells, L02 cells, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83831-5-RR is PROSC Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PROSC fusion protein Ag18369
• Full Name: proline synthetase co-transcribed homolog (bacterial)
• Calculated Molecular Weight: 275 aa, 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC012334
• Gene Symbol: PROSC
• Gene ID (NCBI): 11212
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human Pyridoxal 5′-phosphate-binding protein (PLPBP; previously proline synthase cotranscribed homolog, PROSC) was first described in 1999 and was demonstrated to be ubiquitously expressed in many tissues. PROSC is ubiquitously expressed in human tissues and is highly conserved throughout evolution. PROSC deficiency could therefore markedly increase free cellular PLP levels (PMID: 31825581, 27912044).