PROSC Polyclonal antibody

PROSC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25154-1-AP

Synonyms

PLPBP, Pyridoxal phosphate-binding protein, Pyridoxal phosphate homeostasis protein, Proline synthase co-transcribed bacterial homolog protein, PLP homeostasis protein

---

Validation Data Gallery

• 

  WB analysis using 25154-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25154-1-AP (PROSC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 25154-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25154-1-AP (PROSC antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 25154-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 25154-1-AP (PROSC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 25154-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25154-1-AP (PROSC Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 25154-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25154-1-AP (PROSC Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of L02 using 25154-1-AP

  Immunofluorescent analysis of L02 cells using 25154-1-AP (PROSC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

25154-1-AP targets PROSC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PROSC fusion protein Ag18369
• Full Name: proline synthetase co-transcribed homolog (bacterial)
• Calculated Molecular Weight: 275 aa, 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC012334
• Gene Symbol: PROSC
• Gene ID (NCBI): 11212
• RRID: AB_2879929
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human Pyridoxal 5′-phosphate-binding protein (PLPBP; previously proline synthase cotranscribed homolog, PROSC) was first described in 1999 and was demonstrated to be ubiquitously expressed in many tissues. PROSC is ubiquitously expressed in human tissues and is highly conserved throughout evolution. PROSC deficiency could therefore markedly increase free cellular PLP levels (PMID: 31825581, 27912044).

Publications

Species	Application	Title
human	WB	Brain; PLPHP deficiency: clinical, genetic, biochemical, and mechanistic insights.; Authors - Devon L Johnstone
mouse	WB	Int J Mol Sci; Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling; Authors - Jana Key