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CD133-1,2 Polyclonal antibody

CD133-1,2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 19946-1-AP

Synonyms

AC133, Antigen AC133, CD133, CORD12, MCDR2, MSTP061, PROM1, PROM1 1, PROM1 1,2, PROM1 1,2,3,5,7, PROM1 1,6,7, PROM1 2, PROM1 2,3,4,5, prominin 1, Prominin like protein 1, PROML1, RP41, STGD4

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-29 cells using CD133-1,2 Polyclonal antibody (19946-1-AP)

    WB analysis of HT-29 using 19946-1-AP

    HT-29 cells were subjected to SDS PAGE followed by western blot with 19946-1-AP (CD133-1,2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Caco-2 cells using CD133-1,2 Polyclonal antibody (19946-1-AP)

    WB analysis of Caco-2 using 19946-1-AP

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 19946-1-AP (CD133-1,2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Y79 cells using CD133-1,2 Polyclonal antibody (19946-1-AP)

    IP experiment of Y79 using 19946-1-AP

    IP result of anti-CD133-1,2 (IP:19946-1-AP, 4ug; Detection:19946-1-AP 1:300) with Y79 cells lysate 2000ug.

Tested Applications

Positive WB detected inHT-29 cells, Caco-2 cells
Positive IP detected inY79 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19946-1-AP targets CD133-1,2 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name prominin 1
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 110-120 kDa
GenBank Accession NumberNM_006017
Gene Symbol CD133
Gene ID (NCBI) 8842
RRIDAB_10644275
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors. This antibody recognizes transcript variant 1,2 of CD133.

Protocols

Product Specific Protocols
WB protocol for CD133-1,2 antibody 19946-1-APDownload protocol
IP protocol for CD133-1,2 antibody 19946-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols