Y79 cells were subjected to SDS PAGE followed by western blot with 19945-1-AP (PROM1-1,2,3,5,7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
Y79 cells were subjected to SDS PAGE followed by western blot with 19945-1-AP (PROM1-1,2,3,5,7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 19945-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 19945-1-AP (PROM1-1,2,3,5,7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
mouse lung tissue were subjected to SDS PAGE followed by western blot with 19945-1-AP (PROM1-1,2,3,5,7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
IP experiment of Y79 using 19945-1-AP
IP result of anti-CD133-1,2,4,7 (IP:19945-1-AP, 4ug; Detection:19945-1-AP 1:300) with Y79 cells lysate 2000ug.
IP result of anti-CD133-1,2,4,7 (IP:19945-1-AP, 4ug; Detection:19945-1-AP 1:300) with Y79 cells lysate 2000ug.
IHC staining of human gliomas using 19945-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19945-1-AP (CD133-1,2,4,7 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19945-1-AP (CD133-1,2,4,7 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 19945-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19945-1-AP (CD133-1,2,4,7 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19945-1-AP (CD133-1,2,4,7 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
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human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:200-1:1000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
19945-1-AP targets CD133-1,2,4,7 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors. This antibody recognizes transcript variant 1,2,4,7 (UNIPROT has updated the info of isoforms)of CD133.
Y79 cells were subjected to SDS PAGE followed by western blot with 19945-1-AP (PROM1-1,2,3,5,7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 19945-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 19945-1-AP (PROM1-1,2,3,5,7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human gliomas using 19945-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19945-1-AP (CD133-1,2,4,7 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 19945-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19945-1-AP (CD133-1,2,4,7 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of Y79 using 19945-1-AP
IP result of anti-CD133-1,2,4,7 (IP:19945-1-AP, 4ug; Detection:19945-1-AP 1:300) with Y79 cells lysate 2000ug.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CD133-1,2,4,7 Polyclonal antibody
Catalog Number
19945-1-AP
Citations
-
Dilutions
WB : 1:200-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500
Applications
WB, IP, IHC, ELISA
Reactivity
human, mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
