PRM1 Polyclonal antibody, PBS Only

PRM1 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15697-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 15697-1-AP (same clone as 15697-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 15697-1-AP (PRM1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15697-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 15697-1-AP (same clone as 15697-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15697-1-AP (PRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15697-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat testis using 15697-1-AP (same clone as 15697-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 15697-1-AP (PRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15697-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 15697-1-AP (same clone as 15697-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15697-1-AP (PRM1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15697-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 15697-1-AP (same clone as 15697-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PRM1 antibody (15697-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15697-1-PBS in a different storage buffer formulation.

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Product Information

15697-1-PBS targets PRM1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRM1 fusion protein Ag8323
• Full Name: PRM1
• Calculated Molecular Weight: 51 aa, 6 kDa
• Observed Molecular Weight: 13-20 kDa
• GenBank Accession Number: BC003673
• Gene Symbol: PRM1
• Gene ID (NCBI): 5619
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PRM1 is a 51 amino acid protein. PRM1 substitutes for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex. PRM1 is expressed in testis. The calculated molecular weight of PRM1 is 9 kDa, but Phosphorylated protein is about 20 kDa. (PMID: 24818823)