PKC Gamma Polyclonal antibody

PKC Gamma Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14364-1-AP


PKC gamma, PKCC, PKCG, PRKCG, Protein kinase C gamma type, protein kinase C, gamma, SCA14

Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human cerebellum tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, SH-SY5Y cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14364-1-AP targets PKC Gamma in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKC Gamma fusion protein Ag5681
Full Name protein kinase C, gamma
Calculated Molecular Weight 78 kDa
Observed Molecular Weight 76-80 kDa
GenBank Accession NumberBC047876
Gene Symbol PRKCG
Gene ID (NCBI) 5582
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC gamma is a neuron-specific member of the classical PKCs and is activated and translocated to subcellular regions as a result of various stimuli, including diacylglycerol synthesis, increased intracellular Ca(2+) and phorbol esters. Defects in this protein have been associated with spinocerebellar ataxia type 14 (SCA14), an autosomal dominant neurodegenerative disease.


Product Specific Protocols
WB protocol for PKC Gamma antibody 14364-1-APDownload protocol
IHC protocol for PKC Gamma antibody 14364-1-APDownload protocol
IF protocol for PKC Gamma antibody 14364-1-APDownload protocol
IP protocol for PKC Gamma antibody 14364-1-APDownload protocol
FC protocol for PKC Gamma antibody 14364-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Sci Adv

Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.

Authors - Odetta Antico


Perinatal exposure to nonylphenol impairs dendritic outgrowth of cerebellar Purkinje cells in progeny.

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Cell Oncol (Dordr)

The SLITRK4-CNPY3 axis promotes liver metastasis of gastric cancer by enhancing the endocytosis and recycling of TrkB in tumour cells

Authors - Yao-Qi Zhou

J Neurosci

Dendritic Spine Dynamics after Peripheral Nerve Injury: An Intravital Structural Study.

Authors - Curtis A Benson

J Ethnopharmacol

Analgesic effects and possible mechanisms of iridoid glycosides from Lamiophlomis rotata (Benth.) Kudo in rats with spared nerve injury.

Authors - Yanan Zheng

Inflamm Bowel Dis

LRRC19 Promotes Permeability of the Gut Epithelial Barrier Through Degrading PKC-ζ and PKCι/λ to Reduce Expression of ZO1, ZO3, and Occludin.

Authors - Xiaomin Su


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Chiara (Verified Customer) (10-04-2021)

Clear single band

  • Applications: Western Blot
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: mesenchymal Stem cell