Validation Data Gallery
|Positive WB detected in||human testis tissue, human liver tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human colon cancer tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
55388-1-AP targets PKA C-alpha specific in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, sheep|
|Host / Isotype||Rabbit / IgG|
|Full Name||protein kinase, cAMP-dependent, catalytic, alpha|
|Calculated molecular weight||41 kDa|
|Observed molecular weight||38-43 kDa|
|GenBank accession number||NM_002730|
|Gene ID (NCBI)||5566|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
PRKACA, also named as PKACA and PKA C-alpha, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It phosphorylates a large number of substrates in the cytoplasm and the nucleus. This antibody is specific to PRKACA.
|Product Specific Protocols|
|WB protocol for PKA C-alpha specific antibody 55388-1-AP||Download protocol|
|IHC protocol for PKA C-alpha specific antibody 55388-1-AP||Download protocol|
|IP protocol for PKA C-alpha specific antibody 55388-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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