PRC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PRC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 15617-1-AP

Synonyms

ASE1

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Validation Data Gallery

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  WB analysis of MCF-7 using 15617-1-AP

  WB result of PRC1 antibody (15617-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRC1 transfected MCF-7 cells.

• 

  WB analysis using 15617-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15617-1-AP (PRC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15617-1-AP

  IP result of anti-PRC1 (IP:15617-1-AP, 4ug; Detection:15617-1-AP 1:300) with HEK-293 cells lysate 1480ug.

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  IHC staining of human breast cancer using 15617-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15617-1-AP (PRC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15617-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15617-1-AP (PRC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15617-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (15617-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HEK-293 cells, HeLa cells, K-562 cells, Raji cells, SH-SY5Y cells, C6 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 10 publications below
• CoIP: See 1 publications below

Product Information

15617-1-AP targets PRC1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRC1 fusion protein Ag8028
• Full Name: protein regulator of cytokinesis 1
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC003138
• Gene Symbol: PRC1
• Gene ID (NCBI): 9055
• RRID: AB_2169527
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively. The dual bands between 66-72 kDa detected by this antibody may represent different forms of PRC1.

Publications

Species	Application	Title
human	WB,IHC	Nat Commun; Therapeutic targeting of the PLK1-PRC1-axis triggers cell death in genomically silent childhood cancer.; Authors - Jing Li
mouse	IF	Dev Cell; Distinct classes of lagging chromosome underpin age-related oocyte aneuploidy in mouse.; Authors - Aleksandar I Mihajlović
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song
human	IF	PLoS Biol; The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.; Authors - Umut Batman
human	WB,IF,CoIP	Eur J Med Chem; Identification of naphthalimide-derivatives as novel PBD-targeted polo-like kinase 1 inhibitors with efficacy in drug-resistant lung cancer cells; Authors - Pingping Li
mouse	WB	Development; Aurora kinase B inhibits aurora kinase A to control maternal mRNA translation in mouse oocytes.; Authors - Mansour Aboelenain