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PRC1 Polyclonal antibody

PRC1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, rat and More (1)





Cat no : 15617-1-AP



Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells, HeLa cells, K-562 cells, Raji cells, SH-SY5Y cells, C6 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15617-1-AP targets PRC1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRC1 fusion protein Ag8028
Full Name protein regulator of cytokinesis 1
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC003138
Gene Symbol PRC1
Gene ID (NCBI) 9055
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively. The dual bands between 66-72 kDa detected by this antibody may represent different forms of PRC1.


Product Specific Protocols
WB protocol for PRC1 antibody 15617-1-APDownload protocol
IHC protocol for PRC1 antibody 15617-1-APDownload protocol
IF protocol for PRC1 antibody 15617-1-APDownload protocol
IP protocol for PRC1 antibody 15617-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

Therapeutic targeting of the PLK1-PRC1-axis triggers cell death in genomically silent childhood cancer.

Authors - Jing Li

Dev Cell

Distinct classes of lagging chromosome underpin age-related oocyte aneuploidy in mouse.

Authors - Aleksandar I Mihajlović


Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.

Authors - Hongyuan Song

PLoS Biol

The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.

Authors - Umut Batman


Aurora kinase B inhibits aurora kinase A to control maternal mRNA translation in mouse oocytes.

Authors - Mansour Aboelenain

Front Cell Dev Biol

PRC1 and RACGAP1 are Diagnostic Biomarkers of Early HCC and PRC1 Drives Self-Renewal of Liver Cancer Stem Cells.

Authors - Shixin Liao