PPP2R5E Polyclonal antibody

PPP2R5E Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 23885-1-AP

Synonyms

PPP2R5E

---

Validation Data Gallery

• 

  WB analysis of HeLa using 23885-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23885-1-AP (PPP2R5E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 23885-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 23885-1-AP (PPP2R5E Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 23885-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 23885-1-AP (PPP2R5E Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 23885-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 23885-1-AP (PPP2R5E antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 23885-1-AP is PPP2R5E Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPP2R5E fusion protein Ag20891
• Full Name: protein phosphatase 2, regulatory subunit B', epsilon isoform
• Calculated Molecular Weight: 467 aa, 55 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC092477
• Gene Symbol: PPP2R5E
• Gene ID (NCBI): 5529
• RRID: AB_2879350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Protein Cell; Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice; Authors - He-Zhou Huang