PPP2R3A Polyclonal antibody

PPP2R3A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14720-1-AP

Synonyms

PPP2R3, PPP2R3A, PR130, PR72

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Validation Data Gallery

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  WB analysis of PC-3 using 14720-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14720-1-AP (PPP2R3A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14720-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14720-1-AP (PPP2R3A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: PC-3 cells, MCF-7 cells
• Positive IHC detected in: human skin tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

14720-1-AP targets PPP2R3A in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPP2R3A fusion protein Ag6450
• Full Name: protein phosphatase 2 (formerly 2A), regulatory subunit B'', alpha
• Calculated Molecular Weight: 130 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC065531
• Gene Symbol: PPP2R3A
• Gene ID (NCBI): 5523
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP2R3A(Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha), also named as PPP2R3, has 2 isoforms produced by alternative splicing named as PR130 and PR72. The protein contains stretches of basic residues that may be putative nuclear localization signals, and a cluster of proline residues in an N-terminal region common to both isoforms that the isoform PR130 but not PR72 is variably expressed in a number of tumor cell lines. The PR72 and PR130 cDNAs encode 72 and 130 kDa proteins when expressed in rabbit reticulocyte lysates.(PMID:8392071).

Publications

Species	Application	Title
human	WB,IF	Cell Biosci; GREM1/PPP2R3A expression in heterogeneous fibroblasts initiates pulmonary fibrosis.; Authors - Xiaoni Shi
human	WB	Cell Signal; PP2A-AMPKα-HSF1 axis regulates metal-induced HSP expression and ROS clearance.; Authors - Xiao-Nian Zhu