OCT2 Polyclonal antibody

OCT2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10867-2-AP

Synonyms

Oct 2, OCT2, Octamer binding protein 2, OTF 2, OTF2, POU class 2 homeobox 2, POU2F2

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 10867-2-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10867-2-AP (OCT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 10867-2-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10867-2-AP (OCT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 10867-2-AP

  IP result of anti-OCT2 (IP:10867-2-AP, 4ug; Detection:10867-2-AP 1:300) with NIH/3T3 cells lysate 1200ug.

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  IHC staining of human lymphoma using 10867-2-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10867-2-AP (OCT2 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, mouse ovary tissue
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• ChIP: See 1 publications below

Product Information

10867-2-AP targets OCT2 in WB, IHC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OCT2 fusion protein Ag0955
• Full Name: POU class 2 homeobox 2
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC006101
• Gene Symbol: POU2F2
• Gene ID (NCBI): 5452
• RRID: AB_2167080
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POU2F2, also named as OCT2, is a 479 amino acid protein, which belongs to the POU transcription factor family. The Pit-Oct-Unc (POU) family of transcription factors includes many proteins that have been grouped in seven distinct classes according to function and expression containing proteins such as the neuronally expressed Pit proteins in class I, the ubiquitously expressed Oct-1 and tissue-specific Oct-2 in the second category, the well-known pluripotency marker Oct-4 which belongs to class V. Oct-1 (POU2f1) is a ubiquitous factor present in a wide range of cell types while Oct-2 (POU2f2) expression has been found confined to B lymphocytes and neuronal cells. Oct-2 has been implicated in B cell development and regulation of B lymphocyte-specific genes. POU2F2 exists various isoforms with MV 43, 50, and 62 kDa.

Publications

Species	Application	Title
human	WB,ChIP	Mol Cell; IκBζ is a dual-use coactivator of NF-κB and POU transcription factors; Authors - Aktan Alpsoy