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  • Featured Product
  • KD/KO Validated

POP1 Polyclonal antibody, PBS Only

POP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12029-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POP1 Polyclonal antibody (12029-1-AP)

    WB analysis using 12029-1-AP (same clone as 12029-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12029-1-AP (POP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using POP1 Polyclonal antibody (12029-1-AP)

    WB analysis of HeLa using 12029-1-AP (same clone as 12029-1-PBS)

    WB result of POP1 antibody (12029-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-POP1 transfected HeLa cells. This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using POP1 Polyclonal antibody (12029-1-AP)

    IP experiment of HeLa using 12029-1-AP (same clone as 12029-1-PBS)

    IP result of anti-POP1 (IP:12029-1-AP, 4ug; Detection:12029-1-AP 1:8000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using POP1 Polyclonal antibody (12029-1-AP)

    IHC staining of human ovary cancer using 12029-1-AP (same clone as 12029-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12029-1-AP (POP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using POP1 Polyclonal antibody (12029-1-AP)

    IF Staining of HepG2 using 12029-1-AP (same clone as 12029-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POP1 antibody (12029-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

Product Information

12029-1-PBS targets POP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POP1 fusion protein Ag2660
Full Name processing of precursor 1, ribonuclease P/MRP subunit (S. cerevisiae)
Calculated Molecular Weight 1024 aa, 115 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC011529
Gene Symbol POP1
Gene ID (NCBI) 10940
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Processing of Precursor 1 (POP1), a core component of RNase subunits, is an autoantigen in patients with connective tissue disease and is involved in suppressing inflammation.