PON1 Polyclonal antibody

PON1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 18155-1-AP

Synonyms

A esterase 1, Aromatic esterase 1, ESA, K 45, paraoxonase 1, PON, PON 1, PON1, Serum aryldialkylphosphatase 1

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Validation Data Gallery

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  WB analysis of human plasma using 18155-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 18155-1-AP (PON1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 18155-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18155-1-AP (PON1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 18155-1-AP

  IP result of anti-PON1 (IP:18155-1-AP, 4ug; Detection:18155-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human liver using 18155-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18155-1-AP (PON1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 18155-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18155-1-AP (PON1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human plasma, mouse liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below

Product Information

18155-1-AP targets PON1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PON1 fusion protein Ag12934
• Full Name: paraoxonase 1
• Calculated Molecular Weight: 355 aa, 40 kDa
• Observed Molecular Weight: 35 kDa, 40-45 kDa
• GenBank Accession Number: BC074719
• Gene Symbol: PON1
• Gene ID (NCBI): 5444
• RRID: AB_2166315
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PON1, also named PON and K-45, belongs to the paraoxonase family. PON1 hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. It is capable of hydrolyzing a broad spectrum of organophosphate substrates and several aromatic carboxylic acid esters. PON1 may mediate enzymatic protection of low-density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. PON1 was identified as a triple band in a range of approximately 35-40 kDa. For glycosylated, the MW of PON1 is migrated 42-45kd, while the 35 kDa band represents the unglycosylated form (PMID: 17906223, 32002976, 21852972). The antibody has cross-reaction to PON2 and PON3.

Publications

Species	Application	Title
human	WB	Cell Death Dis; GPx1 is involved in the induction of protective autophagy in pancreatic cancer cells in response to glucose deprivation.; Authors - Qingcai Meng
human	IF	Front Cell Dev Biol; Significance of Liver Zonation in Hepatocellular Carcinoma.; Authors - Shizhe Yu
mouse	WB,IP	Cell Mol Neurobiol; PGC-1α Inhibits Schwann Cell Dedifferentiation and Delays Peripheral Nerve Degeneration by Targeting PON1; Authors - Ying Zou
mouse	WB,IF	Respir Res; Loss of interferon regulatory factor-1 prevents lung fibrosis by upregulation of pon1 expression; Authors - Aiyuan Zhou
mouse	WB,IF	Biomed Pharmacother; GYY4137, a H2S donor, ameliorates kidney injuries in diabetic mice by modifying renal ROS-associated enzymes; Authors - Xueqi Chen
human,mouse	IHC,ELISA	Elife; Multi-targeted therapy resistance via drug-induced secretome fucosylation; Authors - Mark Borris D Aldonza