|Positive WB detected in||HeLa cells|
|Positive IHC detected in||human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15645-1-AP targets POM121 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.
|Tested Reactivity||human, rat, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||POM121 fusion protein Ag8177|
|Full Name||POM121 membrane glycoprotein (rat)|
|Calculated molecular weight||984 aa, 99 kDa|
|Observed molecular weight||140-150 kDa|
|GenBank accession number||BC008794|
|Gene ID (NCBI)||9883|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
POM121 is an integral membrane protein that localizes to the central spoke ring complex and participates in anchoring the nuclear pore complex to the nuclear envelope. Catalog#15645-1-AP do well in identifying the nuclear envelope in immunofluorescence experiments.
Heritable transcriptional defects from aberrations of nuclear architecture
Interactions between ALS-linked FUS and nucleoporins are associated with defects in the nucleocytoplasmic transport pathway.
A tubule-sheet continuum model for the mechanism of nuclear envelope assembly
Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.
POM121 promotes proliferation and metastasis in non-small-cell lung cancer through TGF-β/SMAD and PI3K/AKT pathways
Cardiovirus leader proteins retarget RSK kinases toward alternative substrates to perturb nucleocytoplasmic traffic
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
bing (Verified Customer) (08-06-2019)
Works great in Western Blot, I use it for Hela and Vero cells lysate, go dilution 1:5000 in 5% milk.
Yen-Chen (Verified Customer) (12-03-2018)