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POLR3A Polyclonal antibody, PBS Only

POLR3A Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28842-1-PBS

Synonyms

RNA polymerase III subunit C1, hRPC155, EC:2.7.7.6, DNA-directed RNA polymerase III subunit RPC1, DNA-directed RNA polymerase III subunit A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POLR3A Polyclonal antibody (28842-1-AP)

    WB analysis using 28842-1-AP (same clone as 28842-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28842-1-AP (POLR3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28842-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using POLR3A Polyclonal antibody (28842-1-AP)

    IF Staining of A431 using 28842-1-AP (same clone as 28842-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using POLR3A antibody (28842-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 28842-1-PBS in a different storage buffer formulation.

Product Information

28842-1-PBS targets POLR3A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLR3A fusion protein Ag29036
Full Name polymerase (RNA) III (DNA directed) polypeptide A, 155kDa
Calculated Molecular Weight 1390 aa, 156 kDa
Observed Molecular Weight155 kDa
GenBank Accession NumberBC041089
Gene Symbol POLR3A
Gene ID (NCBI) 11128
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

POLR3A encodes the largest subunit of human RNA polymerase III (RPC1), which contributes to the catalytic activity of Pol III and is part of the active centre of the polymerase. Pol III is responsible for the transcription of untranslated RNAs (PMID: 25339210). Bi-allelic missense variants in POLR3A have been associated with phenotypes distinct from WRS: hypogonadotropic hypogonadism and hypomyelinating leukodystrophy with or without oligodontia (PMID: 30414627).